منابع مشابه
Refilling of caffeine-sensitive intracellular calcium stores in bovine airway smooth muscle cells.
The goal of this study was to assess the mechanisms by which the caffeine-sensitive calcium stores of airway smooth muscle cells are refilled. Bovine trachealis cells were loaded with fura 2-AM (0.5 μM) for imaging of cytosolic calcium concentrations ([Ca2+]i) in the inner cytosol. After a first stimulation (S1) with caffeine, the response to a second stimulation (S2) depended on the presence o...
متن کاملA calcium-induced calcium influx factor, nitric oxide, modulates the refilling of calcium stores in astrocytes.
The roles of nitric oxide are primarily undefined in astrocytes, cells that are active partners in synaptic transmission. Because nitric oxide synthases are present in astrocytes, we imaged the formation of nitric oxide in cultured murine cortical astrocytes using DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate). We demonstrated that physiological concentrations of ATP induced...
متن کاملRole of proton gradients and vacuolar H+-ATPases in the refilling of intracellular calcium stores in exocrine cells
Numerous hormones and neurotransmitters activate cells by increasing cytosolic calcium concentration ([Ca(2+)](i)), a key regulatory factor for many cellular processes. A pivotal feature of these Ca(2+) signals is the release of Ca(2+) from intracellular stores, which is followed by activation of extracellular calcium influx, allowing refilling of the stores by SERCA pumps associated with the e...
متن کاملIntracellular Calcium Stores and Sodium- Calcium Exchanger in Cardiac Myocytes
Cytosolic Ca2+, [Ca]I , has a key role in intracellular signalling during excitation-contraction coupling (E-C coupling) in cardiac myocytes. The sarcoplasmic reticulum (SR) is a main intracellular Ca2+ store and the Na+-Ca2+ exchanger (NaCaX) is a major mechanism to extrude Ca2+ for balancing the Ca2+ influx via L-type Ca2+ channels during excitation. Furthermore, [Ca]I also affects the config...
متن کاملImaging of intracellular calcium stores in single permeabilized lens cells.
Intracellular Ca2+ stores in permeabilized sheep lens cells were imaged with mag-fura 2 to characterize their distribution and sensitivity to Ca2+-releasing agents. Inositol 1,4,5-trisphosphate (IP3) or cyclic ADP-ribose (cADPR) released Ca2+ from intracellular Ca2+ stores that were maintained by an ATP-dependent Ca2+ pump. The IP3 antagonist heparin inhibited IP3- but not cADPR-mediated Ca2+ r...
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ژورنال
عنوان ژورنال: Drug Discovery Today: Disease Mechanisms
سال: 2010
ISSN: 1740-6765
DOI: 10.1016/j.ddmec.2010.08.004